I recently received a grant from the Animal Behavior Society to buy enzyme immunoassay kits. These kits will be used to measure how cortisol (a “stress hormone”) concentrations influence the ability of trematode parasites to infect California killifish (i.e., are killifish with higher cortisol levels more likely to become infected by their trematode parasites?), and to measure how cortisol concentrations change after infection. Why I’m asking these questions is a good story for another day. Today I want to tell you about how the kits I’ll be purchasing work, because they’re AWESOME:
Below is a picture of a finished plate. In this case, the second and third columns were used as standards. If you look carefully, you can see that they start very faint, and get more bright as you move towards the bottom. This is because more of the kit hormone (bound to the tracer) has been added to each well as you progress down the plate.